Evaluation of antioxidant activity of some plant extracts. International journal of modern chemistry and applied science. Issn phytochemical analysis and free radical scavenging. The antioxidant activity of extracts was evaluated according to the amount of malonaldehyde mda formed by the feso 4induced oxidation of linoleic acid and a high pufa oil. Both the crude plant extract and the alumina nps showed a strandbreaking effect on bacterial dna figure 5. Green synthesis of silver nanoparticles was successfully done using cleome viscosa plant extract, simple, rapid, ecofriendly and a cheaper method. However, after acting of feno 3 within 20min, the suspension showed the change in color and turned dark brown after 4 hours of incubation at room temperature.
Herbal medicine, phytochemical compounds, traditional therapy, plant extracts 1. The obtained agnps were characterized by uv, ftir, xrd, fesemedax and tem analysis. The values for dpph, frap, ferrous chelation and inhibition of. Digest journal of nanomaterials and biostructures, 43. Isolation and characterization of bioactive compounds from. Comparative study of antioxidant properties and total. Bello abstract phytochemicalsare active secondary plant metabolites responsible for most of the claimed medicinal activities of plants. Balasubramanian2 1department of chemistry, mahendra engineering college, namakkal637503, india. Screening of in vitro antioxidant activity of methanolic leaf. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. The plant aqueous, ethanolic, acetone and methanolic extracts were screened for the presence of the phytochemical classes by using the standard following methods. Here we have developed a rapid, ecofriendly and convenient green method for the synthesis of silver nanoparticles from silver nitrate using leaf extracts of three indian medicinal, namely.
Antioxidant activity of extracts from calocedrus formosana. Free radicals scavenging activity and reducing power of two algerian sahara medicinal plants extracts abderrahim benslama and abdenassar harrar abstract the aim of this study is to evaluate antioxidant activity of aqueous aq. The most common compound in all the plant extracts was catecholics. In these works aoa are given in units % mg, mgml or mgg, that character. In vitro antioxidant activity the free radical scavenging activity of the methanolic leaf and root extracts of the study species, h. Green synthesis of copper nanoparticles by using plant. Professional in supplying plant extract of good quality, nutriherb is a wellknown china plant extract manufacturer and supplier. Certain plant extracts found to have cytotoxic effects1, some showed antioxidant properties 2,3 while a group of plant species effectively showed antimicrobial activities46 and reduced plant diseases like dampingoff and wilt7. The inhibition of the elastase enzyme was also evaluated. Green synthesis of metallic nanoparticles using aqueous. Evaluation of estrogenic activity of plant extracts for the. Okoli and akah, 2004, which may be responsible for.
Preliminary phytochemical screening, quantitative estimation. The hydrogen atom donating ability of the different plant extracts was determined by the decolorization of methanol solution of 2,2diphenyl1picrylhydrazyl dpph. The aim of this study was to investigate the effects of green and zimbro tea and also green coconut water as a reducing and stabilizer agent in gold nanoparticle synthesis. Plantmediated synthesis of silver nanoparticles using. We carefully read and correct essays so that you will receive a paper that is ready antioxidant activity of plant extracts thes for submission or publication. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Screening of plant extracts for antioxidant activity. Activity in some citrus leaves and seeds ethanolic extracts. These nanoparticles were spherical with a size range of 4045 nm, and were characterized using various analysis techniques including. Free radical scavenging activity of plant extracts from calocedrus formosana the free radical scavenging activities of calocedrus formosana extracts were assessed by dpph assay. Evaluation of some plant extracts for standardization and.
From this study, the agnps inhibit the activity of alphaamylase ina dose dependent manner. The diluted working solutions of the test extracts were prepared in methanol. Pdf antioxidant activity of plant extracts from colombian flora. The fresh suspension of plant extracts ware green brown in color. Antioxidant investigations of the ethanol extract along with its fraction using nitric oxide induced free radical assay methods showed good free radical scavenging activity thereby supporting its.
The concentration of extracts in lard varied from 0. In addition, antioxidant properties, such as reducing power and 1,1diphenyl2picrylhydrazyl free radicalscavenging activity, were determined spectrophotometrically. Some investigations have also reported bioinsecticidal effects of plant extracts from. In this analysis 1 mgml of methanol extract of plant was used. Study of total phenolic and flavonoid content, antioxidant. Free radical scavenging by the use of the abts radical. Hence, methane fermentation of maize silage with the addition of tested extracts was performed in eudiometers, and both the biogas volume and methane content in biogas were measured.
Consequently, the search and study of plant extracts that have the highest antiradical and antioxidant activity aoa is a very urgent task. The extraction procedure included 95% vv ethanol extraction and subsequent water treatment. Generally, the presence of different phytochemicals in crude plant extracts has been linked to the detrimental effects of. Saponins, reducing sugar, steroid, flavones and catecholics.
Synthesis and characterization of gold nanoparticles article pdf available in advances in nanoparticles 0503. The cellular extracts from these biological organisms can be used to synthesize nanoparticles of different size and chemical compositions. Antioxidant activity of methanol extracts of different. A significant amount of phenolic acids such as ferulic, caffeic, phydroxybenzoic, protocatechuic, pcoumaric, vanillic, and syringic acids is typical to cereals. This study investigates an efficient and sustainable route of agnp. Free radical scavenging and total antioxidant capacity of. The advantage of using plants in nanoparticles synthesis is that they are easily available, safe to handle and possess a broad variability of metabolites such as antioxidants, nucleotides and vitamins. Thus, there is a need for green chemistry that includes a clean. Preliminary phytochemical screening, quantitative analysis. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Gupta d, chauhan p 2017 green synthesis of silver nanoparticles involving extract of plants of different taxonomic groups. The antimicrobial effects of the plant extracts are sufficient in a way to cater the healing effect.
In the present work we have developed green synthesis of copper nanoparticles via a singlestep, roomtemperature reduction of metal ions using tea leaf extract. Pdf methods for determining the antioxidant activity. Antioxidant activity of plant extracts from colombian flora. Antioxidant activity of natural plant extracts from mate. Okoli and akah, 2004, which may be responsible for the antiinflammatory activity of this plant. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. Synthesis of gold nanoparticles phytoaunps with the use of leaf extracts phytosynthesis is based on the concept of green chemistry. Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpph free radical activity by modified method. The synthesis of metal nanoparticles using plant extracts has developed a rapid, costeffective biosynthetic protocol for bulk synthesis of stable metallic nanoparticles. Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts anjali soni and sheetal sosa abstract the objective of the present study was to evaluate the phytochemical constitution and antioxidant activity of methanolic extract of dried leaves of four medicinally important herbs ocimum sanctum.
Inhibition of microsomal peroxidation data represents, mean sem, for n3 the reducing power of different commercial extracts was performed and showed. Plant extract nutriherb selects raw materials from superior quality producing areas around the world so as to guarantee these sources are efficiency and safety. Antioxidant activity, total phenolic and total flavonoid. Masika3 1department of veterinary physiology, biochemistry and pharmacology, university of ibadan, nigeria. Evaluating the antioxidant activity of different plant. Superoxide radical scavenging activity data represents, mean sem, for n3 figure 3. The ar grade silver nitrate agno3 was purchased from sigmaaldrich chemicals and fresh ocimum leaves were collected from surroundings of tirupati, andhra pradesh, india. All written assignments are antioxidant activity of plant extracts thes thoroughly checked by our editors on grammar, punctuation, structure, transitions, references, and formatting errors.
Extracts from the plant have been reported to show 8090% inhibitory activity against cyclooxygenase, an important enzyme in the prostaglandin biosynthesis pathway mcgaw et al. The activity of medical plant extracts with al 2 o 3. In vitro antioxidant activity of extracts from the leaves of. Our values, commitments, active ingredients natures gifts are plentiful. However, exploration of the plant extracts as the medium of synthesis has heightened interest due to its simplicity, easiness and viable approach and also, the use of plant extracts for the synthesis of nanoparticles does not require elaborate processes 3,4. The development of reliable and ecofriendly metallic nanoparticles needs some consideration on the chemical procedures involved in the synthesis of nanomaterials. Aqueous extracts of different medicinal plants, viz. Both extracts exhibited good activity assessed by the dpph method with bha as standard. Antioxidant properties of the methanol extracts of the leaves and stems of celtis africana adeolu a. In the present study, three plant foods, namely, drumstick leaves moringa oleifera, mint leaves mentha spicata and carrot tuber daucus carota were extracted with ethanol and analyzed for their antioxidant activity. Antibacterial and antifungal activity of terminalia arjuna. Antioxidant activity of herbaceous plant extracts protect. If plant extracts contain antioxidant activity, it may clearup free radicals from the body, and boost the immune system to handle the infection or illness effectively.
The antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpph free radical activity by modified method. Anti implantation activity of the hydroalcoholic tuber. List of important medicinal plants and disease treated. From their results they have suggested that mussaenda frondosa could be pharmaceutically exploited from antioxidant properties. Plot of absorbance fall with respect to the concentration of plant. Radical scavenging activity was calculated using the following formula. Phytochemical analysis and antioxidant activity of gold. Researcharticle preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra intermedia indigenous to balochistan rahmangul,1,2,3 syedumerjan,1,4 syedfaridullah,3 samiullahsherani,1 andnusratjahan3 1facultyofpharmacy,universityofbalochistan,quetta,pakistan 2departmentofhealth,governmentofbalochistan,quetta,pakistan.
Acarbose is a standard drug act as a synthetic inhibitor for enzyme alpha amylase. Antioxidant activity of plant extracts and their effect on. Antioxidant activity of the crude extract was expressed as percentage of dpph free radical inhibition and ic antioxidant activity of medicinal plant 885 figure 2. Numerous works devoted to antioxidant activity of plant extracts are published. Inhibitory effects of the methanolic extracts of the tested plant species ranged from 6. The conventional methods in the production of metallic nanoparticles generate a large amount of hazardous byproducts. Cuprac assay antioxidant activity was determined by the cuprac assay as reported elsewhere. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Aloe vera plant extract with acetone can be used as antimicrobial agents. Test microorganism and antifungal activity colletotrichum gloeosporioides were isolated from carica papaya l. The free radical scavenging capacity of plant extracts was also studied using.
Keywords antioxidant activity, citrus seeds and leaves, h 2 o 2 i. May 30, 2017 antioxidant activity of natural plant extracts from mate ilex paraguariensis, lotus plumule nelumbo nucifera gaertn. Antibacterial activity conclusion in the present time herbal products are considered to be symbols of protection in contrast to the synthetic product. The antioxidant activity of extracts was evaluated according to the amount of malonaldehyde mda formed by the feso 4induced oxidation of linoleic acid and a. Green synthesis and characterization of silver nanoparticles using. For more than thirty years, laboratoires expansciences research and development teams have harnessed the noblest qualities of the plant world to offer you innovative active ingredients and plant extracts.
Antioxidant and phytochemical analysis of ranunculus arvensis. Screening of medicinal plant extracts for antioxidant activity. Girish 1department of chemistry, sahyadri science college, shimoga577201, karnataka, india. Zygophyllaceae and arthrophytum scoparium chenpodiacea, two. Antioxidant activity of some plant extracts of the family. Change in the colour of the solution with time when plant extracts are added to silver salt. The amount of total phenols and flavonoids and the antioxidant activity of leaf, stem, and rhizome methanolic extracts from a commonly consumed anemopsis californica under different storage conditions were investigated. Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq 2 department of plant, soil and agricultural.
Assessment of antioxidant activity of plant extracts by. Because most natural antioxidants and phytochemicals are multifunctional i. Green synthesis of gold nanoparticles using plant extracts. Steroids are used in medicine to treat many diseases. Mahmood from the immunology laboratory of university of malaya. Research article phytochemical analysis of some traditional. Plant extracts acts as a natural reducing and stabilizing. Green synthesis of gold nanoparticles using leaves extract. The antioxidant activity measurements of ethanolic extracts, expressed as trolox equivalent antioxidant capacity teac are presented in table 3. Antimicrobial activities and phytochemical screening of. Antibacterial activity of petroleum ether extract of t. In of this studying the green synthesis of iron oxide nanoparticles fe 2 o 3 nps with celery stalks and green tea leaves extract were used. Screening of in vitro antioxidant activity of methanolic. Dec 19, 2016 total phenolic contents, free radical scavenging activities and antioxidant capacities of origanum onites extracts prepared using different methods were determined.
Biosynthesis of metal nanoparticles extracted from different parts mostly leaf of the plant is the most effective process of synthesis at a very affordable cost. Crataegus douglasii fruit extract 20 as well as various other plant extracts 21 as reducing agent. A brief summary of the experimental conditions for the various methods of. The plant extracts can be possible candidates for drug development. These results suggest that all plant extracts are good inhibitors at low concentrations. Storage effect on phenols and on the antioxidant activity of. Backgroundin recent years, green synthesis of silver nanoparticles agnps has gained much interest from chemists and researchers.
Identification and characterization of plant extracts as a potential indian j. Methanolic extracts of withania somnifera leaves, fruits and. The maximum antifungal activity was observed in acetone extracts 150. Synthesis and characterization of chitosan nanoparticles. The plant hibiscus rosasinensis belongs to the family malvaceae. The aim of the experiment was the evaluation of antioxidant, cytotoxicity, antibacterial, antifungal and cutaneous wound. Green synthesis of silver nanoparticles involving extract. Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order. Distribution of medicinal plants by their state of existence. The effect of various plant extracts was measured on several important oxidation indicators. Total phenolics content of leaf extract also highest 245. Antioxidant activity of some medicinal plant extracts on. Antioxidant properties of the methanol extracts of the leaves. The present study is conducted to discuss how antioxidant activity aoa of extracts from plant leaves impacts on the kinetics of phytosynthesis, the size of the formed nanoparticles, and the stability of their nanosuspensions.
Antioxidant activity of some algerian medicinal plants. Extraction, isolation and characterization of bioactive. The antioxidant activities of methanol extracts of oregano, dittany, thyme, marjoram, spearmint, lavender and basil were tested in lard stored at 75c. The phenolic concentration in plant extracts was determined by singleton et al. Antibacterial and antioxidant activity of traditional. The in vitro antioxidant activity of these extracts was evaluated using 2,2diphenyl1picrylhydrazyl dpph, trolox 6hydroxy2,5,7,8 tetramethylchroman2carboxylic acid equivalent antioxidant capacity teac, 2,2. Cereals contain a wide range of phenolic compounds. The nanoparticles were antimicrobial along with sewage 25. Comparative analysis of the antioxidant and freeradical. Antioxidant activity of plant extracts containing phenolic. To benefit from its bounty we need to respect and protect it. In particular,they have significant free radical scavenging activity. Antioxidant activity of plant extracts from colombian ora 20 02, ma haku nak orn et a l.
Phytochemical composition of ethanolic and aqueous extracts of the leaves, stembark, root, fruits and seeds i. Free radical scavenging activity ic 50 in different methanolic plant extracts. The aqueous extract of fresh leaves of prosopis juliflora was used in the synthesis of silver ag nanoparticles. These data suggest that the combination of in vitro bioassays for estrogenic activity along with isolation and characterization of the active components by lcms is a valid approach for identification of plant extracts beneficial. Silver nanoparticle synthesis from plant extracts and its. The effect of the antioxidant activity of plant extracts. In this concern, indian flora has yet to divulge innumerable sources of costeffective nonhazardous reducing and stabilizing compounds utilized in preparing agnps. The leaf extract also showed a good xanthine oxidase inhibition activity and was double ic 50 value than that of allopurinol a known antioxidant. Different plant seeds extracts of citrus sinensis, hordeum sativum, triticum sativum, canna indica, citrullus vulgaris and capsicum annuum were evaluated for their antioxidant activity by the following methods. The biosynthetic method employing plant extracts has received attention as being simple and viable compared to chemical and physical methods for synthesizing metal nanoparticles 5. For camellia green tea extract, 1 g of leafs was placed in 50 ml of boiling water and.
Phytochemical analysis of the selected five plant extracts. Green synthesis of metallic nanoparticles using aqueous plant extract and their antibacterial activity s. The purpose of the present study was to investigate the antioxidant activity and fructans content in ethanol and water extracts of roots of elecampane inula helenium l. In the previous chapters 3 and 4, it was shown that p. A detailed study was performed on the antioxidant activity of the methanol extract of whole. In addition, plant extracts are also prepared by maceration or percolation of fresh green plants or dried powdered plant material in water andor organic solvent systems. Chemical preparation of iron oxide nanoparticles using. All chemicals and plant extracts precursors used in the synthesis of gold nanoparticles aunps were purchased from suppliers. Wu tw, lee cc, hsu wh, hengel m and shibamoto t department of environmental toxicology, university of california, usa corresponding author. The content of total phenolics in the extracts was determined spectrometrically according to the folin. In vitro antioxidant activity of extracts from the leaves. The nanoparticle showed antimicrobial activity against escherichia coli, proteus mirabilis and shigella flexneri 24. The phytochemical evaluation of bauhinia tomentosa linn has been analysed to a certain extent, the synthesis of metal nanoparticles using various plant parts and the mechanism. The infrared spectrum of the plant extracts, chitosan nanoparticles and chitosan nanoparticlesbotanical extract were obtained by using a confocal micro raman equipped with an atr module of selenium zinc from 650 4000 cm.
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