Alaa2000 under submerged and solid state fermentation. The main objective of the present study is to optimize the production of lasparaginase by fusarium oxysporum using wheat bran under solid state fermentation ssf. Fermentation is the primary technique for the production of various enzymes. Chapter 1a production of lasparaginase by smf 80 time approach for fermentation improvement aravindan and viruthagiri, 2007. Both solid state and submerged fermentation are used for enzyme production. Production of lasparaginase was carried out in three different media, namely, solidstate media, tryptone glucose yeast extract tgy broth and tryptone fructose yeast extract tfy broth the enzyme was purified to near homogeneity by ammonium sulphate precipitation, dialysis, gel filtration on sephadex g100 column and sdspage. An attempt is made in the present study to optimize the cultural conditions for the production of lasparaginase by fusarium oxysporum using submerged fermentation. Lasparaginase catalyzes the hydrolysis of lasparagine amino acid into laspartic acid and ammonia. L asparaginase, produced by various genera under solidstate and submerged cultivation, is an important chemotherapeutic agent used for the treatment of a variety of lymphoproliferative disorders and lymphomas, acute lymphoblastic leukemia in. Solidstate fermentation vs submerged fermentation for the. In this study, the production of high levels of lasparaginase was obtained from aspergillus wentiiin solid state fermentation using agro industrial wastes like coconut oil, palm oil, white and black sesame oil cakes. After the production of lasparaginase were used for purification by chromatography techniques. This is performed to diminish the acrylamide formation which takes place at high temperatures of frying or baking. Mathematical models of bioprocess kinetics facilitate data analysis and provide a strategy for solving problems in industrial fermentation processes chandrasekhar et al.
Ssf is defined as the growth of microorganism on solid substrate which acts as an energy source in the absence of free flowing water. Production, purification and characterization of lasparaginase. Optimization of lasparaginase production by serratia. Finally, partial purification of lasparaginase enzyme using ammonium sulphate precipitation, dialysis, microfiltration and ultrafiltration were carried out to improve the purity of the isolated lasparaginase enzyme. However, solidstate fermentation ssf offers potential benefits compared. Qualitek4 software was used for the designing of oas and for the optimization based analysis of the media for the better production. The plate assay is advantageous as the method is quick and lasparaginase production can be visualized directly from the plates without performing time consuming assays. Lasparaginase is an antineoplastic agent used in the lymphoblastic leukaemia chemotherapy. Solidstate fermentation is a very effective technique as the yield of the product is many times higher when compared to that in smf, and it also offers many other advantages.
Beauveria bassiana, lasparaginase, solid state fermentation, wheat bran, rapid plate assay, marine fungal strain. In this work, sugarcane bagasse scb was used as support for enzyme production in solidstate fermentation ssf by a. Evaluation of fermentation process parameter interactions for the production of l. Pdf production of lasparaginase by aspergillus melleus avnr1. Lasparaginase production has been widely carried out by submerged fermentation smf process. Another interesting technique for asparaginase production is the solidstate fermentation, that allows the use of agroindustrial residues as substrate or support. This production of lasparaginase were produced through solid state fermentation by using carob pod as a substrate. Lasparaginase production by a new isolate bacillus. Production of lasparaginase by aspergillus niveus under. When used as the sole source for growth in ssf, bran of g. Beauveria bassiana mss1841 was used in the present study for lasparaginase production, as an extracellular enzyme by solid state fermentation ssf. Using response surface methodology to improve the l. Pdf production of lasparaginase by fusarium oxysporum.
Lasparaginase is a therapeutic enzyme which plays a vital role in treating different forms of cancer. Grams reaction, motility testing, lactose fermentation, indole production, methyl red, voges proskauer vp reaction, citrate utilization, h 2 s production, catalase and urease tests using a standard protocol holt, 1994. In general, lasparaginase has two applications, one of them is in the nutrition production, where lasparaginase is used to make french fries and potato chips. Ssf lasparaginase is generally produced by using smf method.
Production of lasparaginase by fusarium oxysporum using. Extracellular lasparaginase production in solidstate. Jq673559 was utilized in solid state fermentation for lasparaginase production. Production of lasparaginase by solid state fermentation using. Asparaginase, solid state fermentation, endophytic fungi. Production of l asparaginase, an anticancer agent, from. Ssf is preferred over smf as it is cost effective, ecofriendly and it delivers high yield of enzyme. The optimum ph, temperature, inoculum size and agitation speed for enzyme production were ph 8. Production of extracellular antileukaemic enzyme lasparaginase from marine actinomycetes by solidstate and submerged fermentation.
Effect of initial moisture content on lasparaginase production. Initially, the influence of the variables carbon and. In the present study, an lasparaginase producing soil isolate bacillus aryabhattai strain itbhu02 accession no. Production of lasparaginase by solid state fermentation. Lasparaginase is one of the known drugs in the treatment of cancer especially acute lymphoblatic leukemia. Design aspects of solid state fermentation as applied to microbial bioprocessing. Lasparaginase an anti tumor agent production by fusarium equiseti using solid state fermentation 89 international journal of drug discovery issn. Environmental factors affecting the bacterial lasparaginase production using wheat bran under solid state fermentation abstract lasparaginase has emerged as one of the most important clinically used enzymes as it exhibits chemotherapeutic potential in treatment of acute lymphoblastic leukemia and. Optimization of solid state production of antileukemic. This article reports the production of high levels of lasparaginase from a new isolate of aspergillus niger in solid state fermentation ssf using agrowastes from three leguminous crops bran of cajanus cajan, phaseolus mungo, and glycine max. Production, purification and characterization of l. Lasparaginase is an important enzyme used in the pharmaceutical and food industry, which can be produced by different microorganisms using low cost feedstocks.
Though, smf process is well established, it possesses few disadvantages like huge volume of waste water generation and difficulties in effluent treatment process 18. Screening, optimization of medium and solid state fermentation for lasparaginase production 92 adopted submerged fermentation process. Fractional factorial design of experimentation l18 orthogonal array of taguchi methodology was adopted to optimize nutritional carbon and nitrogen sources, physiological incubation temperature, medium ph, aeration and agitation. Results of the present study revealed lasparaginase production increased exponentially with increase in incubation period and attained maximum 7. Production of asparaginase a solid state fermentation. Selection of most appropriate agroresidual substrate, with optimal media composition and fermentation conditions to maximize the yield of l. Production, purification and characterization of lasparaginase from marine endophytic aspergillus sp.
In the present study a novel strain, streptomyces gulbargensis was explored for the production of extracellular lasparaginase using groundnut cake extract. In the final purification step, the enzyme showed a specific activity of 662. Extracellular lasparaginase production in solidstate fermentation. Production, purification and advance applications of l. The present work deals with production of extracellular lasparaginase from aspergillus terreus mtcc 1782 using bajra seed flour under solid state fermentation process parameters like incubation. In the present study, the fermentation kinetics and the continuous production of lasparaginase by erwinia aroideae are described. The observation made in this study hold great promise for scale up production of lasparaginase from aspergillus terreus mtcc 1782 using bajra seed flour as substrate under solid state fermentation. Request pdf extracellular lasparaginase production in solidstate fermentation by using sugarcane bagasse as support material lasparaginase is an important enzyme used in the pharmaceutical. Production of extracellular antileukaemic enzyme l. Five different moisture contents were taken into account i. L asparaginase production is carried out mainly by submerged fermentation smf.
Production, isolation, and purification of lasparaginase from pseudomonas aeruginosa 50071 using solidstate fermentation. The isolated fungi were screened for lasparaginase production. Different fungal species were isolated and identified using standard manuals, screened initially for the production of extracellular lasparaginase during their growth on czapeks dox medium containing lasparagine as sole source. Initial moisture content is a very important parameter in solid state fermentation and directly affects the enzyme production and its activity. Lasparaginase a biotherapeutic for acute lymphoblastic. Production of extracellular antileukaemic enzyme l asparaginase from marine actinomycetes by solid state and submerged fermentation. International journal of drug discovery lasparaginasean. To overcome this, eukaryotic organisms such as fungi can be used for the production of lasparaginase. Erwinia aroideae nrrlb8, which produces high yields of.
Escherichia coli and erwinia caratovora have been used for the production of the isozymes of asparaginase. Both solidstate and submerged fermentation are used for enzyme production. Pdf production of extracellular antileukaemic enzyme l. This production of lasparaginase were achieved through optimization of fermentation parameters and it showed 6. Printed fermentation kinetics continuous process l. Simplex centroid mixture design to improve lasparaginase production in solidstate fermentation using agroindustrial wastes. Request pdf solidstate fermentation vs submerged fermentation for the production of lasparaginase lasparaginase, an enzyme that catalyzes lasparagine into aspartic acid and ammonia, has. Classical and statistical methods were employed to optimize the process variables and the results were compared. L asparaginase, produced by various genera under solid state and submerged cultivation, is an important chemotherapeutic agent used for the treatment of a variety of lymphoproliferative disorders and lymphomas, acute lymphoblastic leukemia in. Both fungi and bacteria yield an invaluable array of enzymes when fermented on appropriate substrates. L asparaginase production, solid state fermentation, optimization of medium bacillus. The present study focused on utilization of agrowaste byproducts generated from oil mill for lasparaginase enzyme production using serratia marcescens under solid state fermentation. Production of lasparaginase fermentation was carried out following the method described.
Thus, this study has shown the use of cooked chicken bone as food waste and moringa oleifera seeds as natural substance was added as substrate into the production media for lasparaginase production through microbial fermentation. Effect of fermentation period on lasparaginase production by f. Lasparaginase from marine endophytic aspergillus sp. Because of the above disadvantages, solid state fermentation ssf.
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